Biologists Find New Protective Factor Against Excessive Lipid Accumulation in Liver of Obese Mouse

The liver is the vital organ that orchestrates the overall glucose and fat metabolisms in the human body. Disruption of the fat metabolism in the liver will eventually result in hyperglycemia and hyperlipidemia, which are strong risk factors for developing diabetes, hyperlipidemia, and liver cancer. While the outcomes of fat accumulation in the liver have been well established, it remains unclear if the liver possesses any defensive mechanism to work against the damage.

To answer this question, Dr Chan’s team examined the expression of genes in the liver of high fat diet-induced obese mice and found a protein, SH3 domain binding kinase (SBK1), was exclusively elevated in the obese mouse liver. SBK1 is a protein kinase first discovered in 2001, but no follow-up study has been performed to determine its functions in mammals. Hence, the functions of this novel protein remain unknown.

For the first time, Dr Chan’s team found that fatty acid accumulation is an inducer of SBK1 in the mouse liver. They also observed that the mice without the SBK1 gene in their liver, called ‘LSKO (liver-specific SBK1 knockout)’ mice, have higher lipid accumulation and fibrosis in this tissue. Moreover, the LSKO mice displayed uncontrolled hepatic glucose output and higher blood glucose level, and are less sensitive to insulin stimulation than their control cohort, which are strong indicators of diabetes development.

In addition to the animal studies, Dr Chan’s team also utilized cultured cell models to answer how SBK1 gene controlled the lipid metabolism in the liver. They found that SBK1 phosphorylated and enhanced the activity of Nur77, a well-established transcriptional factor, in liver cells to control fatty acid uptake and lipid synthesis. When the SBK1 protein activity was abolished in the liver cells, they took up more fatty acids and developed excessive lipid accumulation that interfered the insulin signaling. Surprisingly, another metabolic hormone in the liver cells, fibroblast growth factor 21 (FGF21), was also reduced when the SBK1 protein was abolished in the cultured liver cells and the LSKO mice. Since FGF21 is an important hormone from the liver to communicate with other peripheral organs like white adipose tissues, the reduced FGF21 hormone production in the LSKO liver thus impairs the communication between the liver and other organs, leading to the development of insulin resistance in other tissues.

To extend their findings to therapeutic application, the research team further tested if manipulating the SBK1 protein activity in the liver could rescue the damaging effect of obesity. Using adenovirus-mediated gene delivery, they transiently increased the amount of SBK1 protein in the mouse’s liver with fructose diet-induced fatty liver disease and found pathological symptoms like liver steatosis, inflammation, etc. hyperlipidemia, and hyperglycemia were all alleviated.

‘Our findings clearly show that SBK1 protein is an important regulator of the lipid metabolism that was neglected before,’ said Dr Chan. ‘We are very excited to see that increasing its activity effectively alleviates the health problems caused by fatty liver. But we still need more studies to fully depict its functional activity so that we can develop SBK1 activator as a new treatment agent for this common liver disease,’ Dr Chan further added.

Sources: 

Palak Ahuja, Xinyi Bi, Chun Fai Ng, Margaret Chui Ling Tse, Miaojia Hang, Brian Pak Shing Pang, Elsie Chit Yu Iu, Wing Suen Chan, Xin Ci Ooi, Anqi Sun, Oana Herlea‐Pana, Zhixue Liu, Xiuying Yang, Baowei Jiao, Xin Ma, Kelvin Ka Lok Wu, Leo Tsz On Lee, Kenneth King Yip Cheng, Chi Wai Lee, Chi Bun Chan. Src homology 3 domain binding kinase 1 protects against hepatic steatosis and insulin resistance through the Nur77‐ fibroblast growth factor 21 pathway. Hepatology, 2022; DOI: 10.1002/hep.32501

The University of Hong Kong. “Biologists find new protective factor against excessive lipid accumulation in liver of obese mouse.” ScienceDaily. ScienceDaily, 19 April 2022. <www.sciencedaily.com/releases/2022/04/220419103243.htm>.

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