A new blood-based diagnostic test would be a major advancement for Parkinson’s disease, which afflicts 10 million people worldwide and is the second-most common neurodegenerative disease after Alzheimer’s.
“Currently, Parkinson’s disease is diagnosed largely based on clinical symptoms after significant neurological damage has already occurred,” said senior author Laurie Sanders, Ph.D.
“A simple blood test would allow us to diagnose the disease earlier and start therapies sooner,” Sanders said.
As a biomarker for their diagnostic tool, Sanders and colleagues focused on DNA damage in the mitochondria. Mitochondria are factories within cells that convert raw energy into a form that powers cells. They contain their own DNA, which can undergo damage separately from the nuclear DNA that encodes most of an organism’s genome.
Using polymerase chain reaction (PCR) technology, the Duke team developed an assay that successfully quantified higher levels of mitochondrial DNA damage in blood cells collected from patients with Parkinson’s disease compared to people without the disease.
The new test also identified high levels of the damaged DNA in the blood samples of people who harbor the genetic mutation LRRK2, which has been associated with an increased risk of the disease. The assay was able to detect patients with Parkinson’s disease with and without LRRK2 mutations.
A further analysis in cells from patients with Parkinson’s disease explored whether the team’s PCR-based test could determine the effectiveness of a therapy that targets the effects associated with LRRK2 mutation.
In these samples, the test identified lower mitochondrial DNA damage in cells treated with a LRRK2 inhibitor compared to samples from patients who did not receive the inhibitor. This suggests the assay could help pinpoint Parkinson’s disease patients who might benefit from LRRK2 kinase inhibitor treatments, even if they do not have a LRRK2 mutation.
“Our hope is that this assay could not only diagnose Parkinson’s disease, but also identify drugs that reverse or halt mitochondrial DNA damage and the disease process,” Sanders said.
Sanders said the research team’s future will include further testing of the assay in samples from patients with the earliest stages of disease, before symptoms develop.
Rui Qi, Esther Sammler, Claudia P. Gonzalez-Hunt, Ivana Barraza, Nicholas Pena, Jeremy P. Rouanet, Yahaira Naaldijk, Steven Goodson, Marie Fuzzati, Fabio Blandini, Kirk I. Erickson, Andrea M. Weinstein, Michael W. Lutz, John B. Kwok, Glenda M. Halliday, Nicolas Dzamko, Shalini Padmanabhan, Roy N. Alcalay, Cheryl Waters, Penelope Hogarth, Tanya Simuni, Danielle Smith, Connie Marras, Francesca Tonelli, Dario R. Alessi, Andrew B. West, Sruti Shiva, Sabine Hilfiker, Laurie H. Sanders. A blood-based marker of mitochondrial DNA damage in Parkinson’s disease. Science Translational Medicine, 2023; 15 (711) DOI: 10.1126/scitranslmed.abo1557
Duke University Medical Center. “New blood test detects a key indicator of Parkinson’s disease.” ScienceDaily. ScienceDaily, 30 August 2023. <www.sciencedaily.com/releases/2023/08/230830151742.htm>.
Photo by Sam Moghadam Khamsen